Mycobacterium tuberculosis resistance to Isoniazid and Rifampicin in a HIV-1 endemic population in western Kenya in 2012-2014

Title: Mycobacterium tuberculosis resistance to Isoniazid and Rifampicin in a HIV-1 endemic population in western Kenya in 2012-2014

Authors: Clement Shiluli, Collins Ouma, John M. Vulule, Jeremiah Khayumbi, Wilfred Murithi, Susan Musau and Albert Okumu

DOI: https://dx.doi.org/10.18535/jmscr/v4i12.51

Abstract

References

Corresponding Author

Abstract

Background: Mono-resistant and multi-drug resistant tuberculosis (MDR-TB) has been enhanced by delays in the identification of resistant strains. However, resistance gene patterns and the extent of mono-resistant TB and MDR-TB in western Kenya is unknown. The objective of the study was to identify cases of mono-resistant TB and MDR-TB in western Kenya.

Methods: Early morning sputum samples were cultured on Mycobacteria growth indicator tubes (MGIT) and incubated at 37 °C. Drug susceptibility testing (DST) using the SIRE® kit was done on ZN smear positive MGIT tubes and line probe assay (LPA) performed to identify specific mutations on the rpo B, kat G and inh A genes. Mutations on discordant samples were confirmed by sequence analysis.

Results: The rpo B H526Y and the kat G S315T1 mutations were common in HIV positive patients (8 % and 18 % respectively) and that the S3I5T1 and S531L was the most common mutation in MDR-TB strains in both HIV positive and negative patients (5 % and 8 % respectively). Binary logistic regression, indicated that RMR TB is associated with HIV status (P = 0.025).

Conclusions: Our findings showed that there is a potential association between RMR TB and HIV-1 status in western Kenya.

Keywords: Tuberculosis, HIV, Drug susceptibility test, Line Probe Assay, MDR-TB, mono resistant TB.

References

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Clement Shiluli

*Correspondence; School of Public Health and Community Development, Department of Biomedical Sciences and Technology, Maseno University, P.O. Box Private Bag, Maseno

Email: clementshiluli@yahoo.com, +254 723 474 168

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Call For Paper Volume 13 Issue 04 April 2025

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