Title: Efficacy of Psidium guajava leaf extract on Streptococcus mutans and Enterococcus faecalis – an in vitro study

Authors: Dr Vidya Venugopal, Dr Gopinath Prakasan, Sandeep P M, Dr Anuradha E Sunil, Dr Archana Mukunda, Dr Steffy Rachel Samuel

 DOI: https://dx.doi.org/10.18535/jmscr/v7i5.121

Abstract

Introduction: The general health of the body is governed largely by oral health. The most common oral diseases are dental caries and gingivitis both of which are caused by micro organisms. Streptococcus mutans and Enterococcus faecalis are the common causative bacteria which are traditionally treated with antibiotics. Many herbal preparations have also known to exhibit potent antibacterial activity.

Objective: To evaluate the antibacterial activity of Psidium guvajava leaf extract on Streptococcus mutans and Enterococcus faecalis

Methods: In vitro study, in which the ethanolic extract of Psidium guajava leaf extract was prepared using solvent extraction apparatus. The extract was then diluted in normal saline to 3 different concentrations. Well diffusion method was used to determine the zone of inhibition of various concentrations of Psidium guajava leaf extract against Streptococcus mutans and Enterococcus faecalis. Zone of inhibition was measured in mm.

Result: At all concentrations guava leaf extract showed zone of inhibition against Streptococcus mutans (S. mutan) and Enterococcus faecalis (E.faecalis). Zone of inhibition increased with concentration of the extract.

Conclusion: Psidium guajava leaf extract demonstrate an anti-microbial property against Streptococcus mutans and Enterococcus faecalis

Keywords: Enterococcus faecalis, Psidium guajava, Streptococcus mutans, zone of inhibition  .

References

  1. R. Isabela, F.S.  Jose and  R.N.S.  Katia, Association of Enterococcus  faecalis  with different forms of periradicular diseases. Journal of Endodontics 2004;30:315 -320.
  2. Pai, L.D. Ahcarya, and N. Udupa, Evaluation of anti-plaque activity of azadirachtaindica leaf extract gel – a 6 week clinical study. Journal of Ethno pharmacology,  90, 2004, 99-103.
  3. Kumbhar AB, Chaudhari P. D. Shaikh K, Dongaonkar C. Modern Approaches For The Treatment Of Periodontitis: A Review. WJPPS 2017;6:628-647.
  4. Ravi and P. Divyashree, psidiumguajava: Review on its potential as an adjunt in treating periodontal disease. Pharmacognosy review, 8(16), 2014; 96-100.
  5. Bipul Biswas, Kimberly Rogers, Fredrick McLaughlin, Dwayne Daniels, and Anand Yadav, Antimicrobial Activities of Leaf Extracts of Guava (Psidiumguajava L.) on Two Gram-Negative and Gram-Positive Bacteria. International journal for microbiology. 2013
  6. V. Kamath, Nair Rahul, C. K. Ashok Kumar, S. Mohana Lakshmi, Psidiumguajava L: A review. International Journal of Green Pharmacy. 2, 2008; 9-12
  7. Barbalho SM, Farinazzi-Machado FM, de AlvaresGoulart R, Brunnati AC, Ottoboni AM. Psidiumguajava (Guava): A plant of multipurpose medicinal plants. Med Aromat Plants. 2012;1:104
  8. Rishika D, Sharma R. An update of pharmacological activity of Psidiumguajava in the management of various disorders. Int J Pharm Sci Res. 2012;3:3577–84.
  9. Kumar P, Ansari SH, Ali J. Herbal remedies for the treatment of periodontal disease: A patent review. Recent Pat Drug Deliv Formul. 2009;3:221–8.

Corresponding Author

Dr Archana Mukunda

Professor, Dept of Oral & Maxillofacial Pathology, Royal Dental College, Chalissery, Kerala, India