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Abstract
One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Accidental releases of petroleum products are of particular concern in the environment. Hydrocarbon components have been known to belong to the family of carcinogens and neurotoxin organic pollutants. Bioremediation is the promising technology for the treatment of these contaminated sites since it is cost-effective and will lead to complete mineralization. In this project hydrocarbon degrading bacteria isolated from hydrocarbon contaminated soil by using nutrient agar medium and mineral salt agar medium supplemented with liquid petrol and diesel which was used as the sole carbon source for bacterial growth was performed. The bacteria was identified by using Bergey’s manual of bacterial classification. The nah gene which responsible for the hydrocarbon degradation was amplified. The sequence has similarity about 90% to nahB gene.
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How to Cite
V, S. P. (2014). Isolation and Identification of Nah-B Gene from Bacteria Isolated from Effluents & Testing its Hydrocarbon Degrading Efficiency. International Journal of Emerging Trends in Science and Technology, 1(09). Retrieved from https://igmpublication.org/ijetst.in/index.php/ijetst/article/view/402
References
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8. Bindu Joshi, Satish Walia (1996).PCR amplification of catechol 2,3-dioxygenase gene sequences from naturally occurring hydrocarbon degrading bacteria isolated from petroleum hydrocarbon contaminated groundwater FEMS Microbiology Ecology Volume 19(1), 5-15.
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10. Chaillan F, Le Fleche A, Bury E, Phantavong Y, Grimont P, Saliot A, Oudot J. (2004) Identification and biodegradation potential of tropical aerobichydro carbon-degrading microorganisms. Res. Microbiol. 155: 587-595.
11. Cowan DA, Strafford W (2007). Metagenomic methods for the identification of active microorganisms and genes in biodegradation processes. In Environmental Microbiology 3rd edition. ASM Press, America Society for Microbiology, USA.
2. Adriano Pinto Mariano; Ana Paula de Arruda Geraldes Kataoka; Dejanira de Franceschi de Angelis; Daniel Marcos Bonotto (2007). Laboratory Study on the Bioremediation of Diesel Oil contaminated soil from a petrol station. Brazilian Journal of Microbiology 38:346-353.
3. Anthony I Okoh (2006). Biodegradation alternative in the cleanup of petroleum hydrocarbon pollutants. Biotechnology and Molecular Biology Review Vol. 1 (2), pp. 38-50.
4. Atlas RM. (1981). Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiol. Rev. 45(1): 180-209.
5. Austin B, Calomiris JJ, Walker JD, Colwell RR. (1977). Numerical taxonomy and ecology of petroleum-degrading bacteria. Appl. Environ Microbiol. 34(1): 60-68.
6. Benkacoker, M. O. and Ekundayo, J. A. (1996). Applicability of evaluating the ability of microbes isolated from an oil spill site to degrade oil. Environmental Monitoring and Assessment 45, 259- 272.
7. Bicca, F.C., L.C. Fleck, M. Antonio and Z. Ayub (1999) Production of biosurfactant by hydrocarbon degrading Rhodococcus ruber and Rhodococcus erythropolis. Rev. de Microbiol., 30, 231-236
8. Bindu Joshi, Satish Walia (1996).PCR amplification of catechol 2,3-dioxygenase gene sequences from naturally occurring hydrocarbon degrading bacteria isolated from petroleum hydrocarbon contaminated groundwater FEMS Microbiology Ecology Volume 19(1), 5-15.
9. Boboye, B., Olukunle, O. F., and Adetuyi, F. C. (2010)Degradative activity of bacteria isolated from hydrocarbon-polluted site in Ilaje, Ondo State, Nigeria African Journal of Microbiology Research Vol. 4(23), pp. 2484-2491,
10. Chaillan F, Le Fleche A, Bury E, Phantavong Y, Grimont P, Saliot A, Oudot J. (2004) Identification and biodegradation potential of tropical aerobichydro carbon-degrading microorganisms. Res. Microbiol. 155: 587-595.
11. Cowan DA, Strafford W (2007). Metagenomic methods for the identification of active microorganisms and genes in biodegradation processes. In Environmental Microbiology 3rd edition. ASM Press, America Society for Microbiology, USA.