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Abstract
ABSTRACT
An in vitro micropropagation system has been developed for Terminalia arjuna Roxb., an important Indian medicinal plant and non mulberry primary food plant of tropical tasar silkworm (Antheria mylitta Drury). Nodal segments obtained from 15-d-old aseptically grown seedlings were used as explants. MS medium containing 1.5 mg/L BAP was found most suitable for culture initiation. Although shoot multiplication was achieved on MS medium containing BAP and Kn, the maximum number of shoots was obtained with 1.5 mg/L BAP. Best rooting response was observed on MS medium salts, 0.6% agar and 0.1 mg/L IBA. Plantlets were hardened initially in culture room conditions and then transferred to misthouse.
Keywords: Axillarys bud proliferation, Arjun, in vitro, rooting, shoot multiplication##plugins.themes.academic_pro.article.details##
How to Cite
Ravi, M., Ramanjaneyulu, P., & Vijaya Bhaskar Rao, A. (2014). Micropropagation of Terminalia Arjuna Roxb., from Nursery Plant Material. International Journal of Emerging Trends in Science and Technology, 1(07). Retrieved from https://igmpublication.org/ijetst.in/index.php/ijetst/article/view/309
References
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3. Amin, M. N.; Jaiswal, V.S. 1983. Rapid clonal propagation of guava through in vitro shoot proliferation on nodal explants of mature trees. Plant Cell Tiss. Organ Cult. 9: 235-243.
4. Avinash, N., Laxman, S.M., Satwinderject, K., Iqbal. S.G., Renu, W. and Sunil, C.K. 2000. Growth suppression of human transformed cells by treatment with bark extracts from a medicinal plant Terminalia arjuna, in vitro cell. Dev. Bio-Animal. 36: 544-547.
5. Ramesh, M., Pawan, U., Prasad, S., Rao, A.V. and Sadanandam, A. 2000 & 2001. Terminalia arjuna: Break-Through in micropropagation. Indian Silk. 17: 25-28.
6. Broom, O.C. and Zimmermann, 1978. In-vitro propagation of blackberry. Hort. Sci. 13: 151-153.
7. Terkey, J., Sinha, A. K., Gangopadyay, A. K., Gangopadyay, and Sinha, B.R.R.P. 2003. Effect of BAP and Kinetin on Terminalia species. Akhil Bhatia Shahtuti Samayik Taknike Seminor, 6-7th March, 2003 at CTR and TI, Ranchi: 1-8.
8. Broom, O.C. and Zimmermann, 1984. Cultures of shoot meristems: fruit plants in cell culture and somatic cells. Genetics of plants. Ed I.K. Vasil, Academic Press Inc. New York.11-121.
9. Sadanandam, A., Ramesh, M., Umate, P., Rao, K.V. 2005. Micropropagation of Terminalia bellerica Roxb. - a sericulture and medicinal plant. In vitro Cell. Dev. Biol. Plant 41:320-323.
10. Bilochi G, 2002. In vitro studies on some medicinal plants of Aravallis in Rajasthan. Ph.D Thesis, Mohanlal Sukhadia University, Udaipur, India.
11. Dhar, U., Upreti, J. 1999. In vitro regeneration of a mature leguminous liana (Bauhinia vahlii White& Arnott). Plant Cell Rep. 18:664-669.
12. Gupta, P. K., Nadgir, A. L., Mascarenhas, A. F., Jagannathan, V. 1980. Tissue culture of forest trees: clonal multiplication of Tectona grandis L. (Teak) by tissue culture. Plant Sci. Litt.17:259-268.
13. Huetteman, C.A.; Preece, J. E. Thidiazuron. 1993. A potent cytokinin for woody plant tissue culture, Plant Cell Tiss. Organ Cult.33: 105-119.
14. Kaur, S., Grover, I.S. and Kumar, S. 2001. Anti mutagenic potential of extracts isolated from Terminalia arjuna J. Environ. Pathol. Toxicol. Oncol. 20: 9-14.
15. Kusumoto, I. T., Nakabayashi, T., Kida, H., Miyashrio Hattori, M., Namba, T. and Shimotohno. 1995. Screening of various plant extracts used in ayurvedic medicine for inhibitory effects on human immunodeficiency virus-I (HIVI) protease. Phytotherapy Research. 9(3): 180.
16. Litz, R. E. 1984. In-vitro responses of adventitious embryos of two polyembryonic Eugenia species. Hort. Sci. 19: 720-722.
17. Nishi, K., Jaiswall U. and Jaiswal V.S. 1998. Introduction of somatic embryogenesis and plants regeneration from leaf callus at Terminalia arjuna. Curr. Sci. 75(10.4): 1052-1085.
18. McNicol, R.J. and Julie Graham. 1990. In vitro regeneration of Rubus from leaf and stem segments. Plant Cell Tissue and Organ Culture 21: 45-50.
19. McClelland, M.A.L. Smith and Z.B. Carothers. 1990. The effect of in vitro and ex vitro root initiation on subsequent microcutting root quality in three woody plants. Plant Cell Tissue Organ Culture 28: 115-123.
20. Murashige, T.; Skoog,F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497.
21. Murashige T. 1974. Plant propagation through tissue culture, Ann Rev Plant Physiol, 25: 135-166.
22. Roy S K, Pal P K& Das A K, 1987. Propagation of a timber tree Terminalia bellerica Roxb. by tissue culture, Bangladesh J Bot. 16: 125-130.
23. Vinolya Kumari, R. and Pullaiah, T. 2000. In vitro morphogenetic studies of Terminalia chebula RETZ., Terminalia pallida Brandis and Samanea saman (JACQ.) MERR. Ph.D thesis, Sri Krishnadevaraya university, Anantapur, AP, India.
24. Pandey, S., Jaisawal, V.S. 2002. Micropropagation of Terminalia arjuna Roxb. from cotyledonary nodes, Indian J. Exp. Biol. 40: 950-953.
25. Purohit S. D, Tak K & Kukda G. 1996. Micropropagation of Wrigtia tomentosa (Roxb.) Roem et Schult, J Sustainable For, 3: 25-35.
26. Tirkey, J., Gangopadyay, A. and Sinha, B.R.R.P. 1999. Effect of anti-oxidants and absorbant on tissue browsing associated metabolism in Terminalia arjuna nodal explants. Curr. Tech.Semi. on non-mulberry sericulture at C.T.R. and T.I., Ranchi. March. 15: 3-9.
27. Gamborg O L., Miller R A & Ojima K. 1968. Nutrient requirement of suspension cultures of Soyabean root cells. Exp Cell Res. 50: 151-158.
28. White P R (Ed) 1963. The cultivation of animal and plant cells (Ronald Press. New York).
29. Murashiege T. 1974. Plant propagation through tissue culture. Ann Rev Plant Phisol, 25: 135-166.
30. Debergh, P.C. and L.J. Macne. 1981. A scheme for commercial propagation of ornamental plants by tissue culture. Sci. Hortic. 14: 335-345.
2. Kumari, N., V. Jaiswall and V.S. Jaiswal. 1999. Induction of somatic embryogenesis and plant regeneration from leaf callus of Terminalia arjuna. Curr. Sci. 75: 1052-1055.
3. Amin, M. N.; Jaiswal, V.S. 1983. Rapid clonal propagation of guava through in vitro shoot proliferation on nodal explants of mature trees. Plant Cell Tiss. Organ Cult. 9: 235-243.
4. Avinash, N., Laxman, S.M., Satwinderject, K., Iqbal. S.G., Renu, W. and Sunil, C.K. 2000. Growth suppression of human transformed cells by treatment with bark extracts from a medicinal plant Terminalia arjuna, in vitro cell. Dev. Bio-Animal. 36: 544-547.
5. Ramesh, M., Pawan, U., Prasad, S., Rao, A.V. and Sadanandam, A. 2000 & 2001. Terminalia arjuna: Break-Through in micropropagation. Indian Silk. 17: 25-28.
6. Broom, O.C. and Zimmermann, 1978. In-vitro propagation of blackberry. Hort. Sci. 13: 151-153.
7. Terkey, J., Sinha, A. K., Gangopadyay, A. K., Gangopadyay, and Sinha, B.R.R.P. 2003. Effect of BAP and Kinetin on Terminalia species. Akhil Bhatia Shahtuti Samayik Taknike Seminor, 6-7th March, 2003 at CTR and TI, Ranchi: 1-8.
8. Broom, O.C. and Zimmermann, 1984. Cultures of shoot meristems: fruit plants in cell culture and somatic cells. Genetics of plants. Ed I.K. Vasil, Academic Press Inc. New York.11-121.
9. Sadanandam, A., Ramesh, M., Umate, P., Rao, K.V. 2005. Micropropagation of Terminalia bellerica Roxb. - a sericulture and medicinal plant. In vitro Cell. Dev. Biol. Plant 41:320-323.
10. Bilochi G, 2002. In vitro studies on some medicinal plants of Aravallis in Rajasthan. Ph.D Thesis, Mohanlal Sukhadia University, Udaipur, India.
11. Dhar, U., Upreti, J. 1999. In vitro regeneration of a mature leguminous liana (Bauhinia vahlii White& Arnott). Plant Cell Rep. 18:664-669.
12. Gupta, P. K., Nadgir, A. L., Mascarenhas, A. F., Jagannathan, V. 1980. Tissue culture of forest trees: clonal multiplication of Tectona grandis L. (Teak) by tissue culture. Plant Sci. Litt.17:259-268.
13. Huetteman, C.A.; Preece, J. E. Thidiazuron. 1993. A potent cytokinin for woody plant tissue culture, Plant Cell Tiss. Organ Cult.33: 105-119.
14. Kaur, S., Grover, I.S. and Kumar, S. 2001. Anti mutagenic potential of extracts isolated from Terminalia arjuna J. Environ. Pathol. Toxicol. Oncol. 20: 9-14.
15. Kusumoto, I. T., Nakabayashi, T., Kida, H., Miyashrio Hattori, M., Namba, T. and Shimotohno. 1995. Screening of various plant extracts used in ayurvedic medicine for inhibitory effects on human immunodeficiency virus-I (HIVI) protease. Phytotherapy Research. 9(3): 180.
16. Litz, R. E. 1984. In-vitro responses of adventitious embryos of two polyembryonic Eugenia species. Hort. Sci. 19: 720-722.
17. Nishi, K., Jaiswall U. and Jaiswal V.S. 1998. Introduction of somatic embryogenesis and plants regeneration from leaf callus at Terminalia arjuna. Curr. Sci. 75(10.4): 1052-1085.
18. McNicol, R.J. and Julie Graham. 1990. In vitro regeneration of Rubus from leaf and stem segments. Plant Cell Tissue and Organ Culture 21: 45-50.
19. McClelland, M.A.L. Smith and Z.B. Carothers. 1990. The effect of in vitro and ex vitro root initiation on subsequent microcutting root quality in three woody plants. Plant Cell Tissue Organ Culture 28: 115-123.
20. Murashige, T.; Skoog,F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497.
21. Murashige T. 1974. Plant propagation through tissue culture, Ann Rev Plant Physiol, 25: 135-166.
22. Roy S K, Pal P K& Das A K, 1987. Propagation of a timber tree Terminalia bellerica Roxb. by tissue culture, Bangladesh J Bot. 16: 125-130.
23. Vinolya Kumari, R. and Pullaiah, T. 2000. In vitro morphogenetic studies of Terminalia chebula RETZ., Terminalia pallida Brandis and Samanea saman (JACQ.) MERR. Ph.D thesis, Sri Krishnadevaraya university, Anantapur, AP, India.
24. Pandey, S., Jaisawal, V.S. 2002. Micropropagation of Terminalia arjuna Roxb. from cotyledonary nodes, Indian J. Exp. Biol. 40: 950-953.
25. Purohit S. D, Tak K & Kukda G. 1996. Micropropagation of Wrigtia tomentosa (Roxb.) Roem et Schult, J Sustainable For, 3: 25-35.
26. Tirkey, J., Gangopadyay, A. and Sinha, B.R.R.P. 1999. Effect of anti-oxidants and absorbant on tissue browsing associated metabolism in Terminalia arjuna nodal explants. Curr. Tech.Semi. on non-mulberry sericulture at C.T.R. and T.I., Ranchi. March. 15: 3-9.
27. Gamborg O L., Miller R A & Ojima K. 1968. Nutrient requirement of suspension cultures of Soyabean root cells. Exp Cell Res. 50: 151-158.
28. White P R (Ed) 1963. The cultivation of animal and plant cells (Ronald Press. New York).
29. Murashiege T. 1974. Plant propagation through tissue culture. Ann Rev Plant Phisol, 25: 135-166.
30. Debergh, P.C. and L.J. Macne. 1981. A scheme for commercial propagation of ornamental plants by tissue culture. Sci. Hortic. 14: 335-345.