Application of Brucella IgM and IgG on Buffy Coat and Serum from Population at Risk, Khartoum, Sudan

Although brucellosis in human is rarely fatal, it can be severely debilitating and disabling. The infection has
a tendency towards chronicity and persistence, becoming a granulomatous disease capable of affecting any
organ systems.
Previous studies showed occurrence of brucellosis among population at risk in Sudan. The aim of this study
was to try to apply serological tests (Rose Bengal Plate test and IgM, IgG flow assay to Buffy coat samples.
Blood samples were collected from 44 persons from population at risk including livestock artificial
inseminators, milkers and other farm workers in Khartoum State, Sudan. Rose Bengal Plate Test (R.B.P.T),
IgM and IgG flow assay were used to analyze the samples of Buffy coat serologically. The results showed
that 19 Buffy coat samples (43.2%) gave positive results with R.B. P. T; one sample (2.3%) gave positive
results with IgM and 3 (6.9 %) gave positive results with IgG. In conclusion Rose Bengal Plate test and IgM,
IgG flow assay can be applied to the Buffy coat; R.B.P.T. being the most reliable.