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Category: Volume 09 Issue 08 August 2021
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Title: Susceptibility of Gram negative isolates to Nitrofurantoin in Urinary Tract Infection at a tertiary health care center in Himachal Pradesh

Authors: Dr Aditya Rana, Dr Subhash Chand Jaryal, Dr Anuradha Sood, Dr Meenakshi Tamrakar, Dr Anjali Sharma

 DOI: https://dx.doi.org/10.18535/jmscr/v9i8.12

Abstract

Introduction: The most common bacterial infection affecting human beings in life time is Urinary tract infections (UTI). Escherichia coli (E.coli) is the most common pathogen in UTIs.  Nitrofurantoin has been used for a long time. In era of increasing resistance and the decline in newly developed antibiotics has escalated engrossment in treatment of bacterial UTI.

Materials & Methods: A descriptive analysis of culture results of urine samples was performed at the Department of Microbiology at DRPGMC Kangra at Tanda from May 2020 to April 2021. Samples were sent in a sterile wide mouth container. The specimens were inoculated on MacConkey agar plates and incubated aerobically at 37°C for 24 hours. Culture plates with colony counts of ≥ 105 cfu/ml were considered positive for UTI. Antimicrobial susceptibility was performed according to CLSI guidelines.

Results: A total of 4286 samples were received, out of which 1244(29.1%) were males and 3042 (70.9%) females. Male to female ratio was 1:2.4. Majority of the cases (71.2%) belonged to the age group 25-45 years. Escherichia coli (E.coli) (50.7%) was the most common pathogen among Gram negative isolates. Maximum sensitivity was seen in E.coli (92%) and maximum resistance was seen in Acinetobacter (90%)

Conclusion: E.coli remained the predominant isolate among gram negative organisms, more commonly in females. The in vitro activity of nitrofurantoin found in the present study suggests that this drug is susceptible to most uropathogens.Regular antimicrobial susceptibility is required to be carried out to see the current pattern of susceptibility.

Keywords: Urinary tract infection, Nitrofurantoin, Sensitivity, Susceptibility, Resistance

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Corresponding Author

Dr Aditya Rana

JR, Department of Microbiology, DRPGMC Kangra at Tanda