Title: Evaluation of the Effectiveness of Different Autoclaves on the Sterilization of Contaminated Rotary Endodontic Files
Authors: Dr Akhil Yadav B, Dr Lekshmy S Devi, Dr Sreeja Sreedhar, Dr Lakshmi Aravind, Dr Siddharth V Nair
DOI: https://dx.doi.org/10.18535/jmscr/v8i10.21
Abstract
The aim of this study was to assess and compare the effectiveness of four accepted methods of endodontic instruments sterilization. The present study was performed on twenty five F2 Protaper files and they were tested for the efficacy of sterilization with different methods namely N class autoclave, B class autoclave, pressure cooker autoclave and glutaraldehyde. The biological indicator used was C albicans. Sterility of fresh files obtained from the manufacturer was also tested in the study. The study showed that the fresh files obtained from the manufacturers are sterile while remnants of microbial colonies was observed in rest of all the sterilization technique. N class autoclave was found to have highest efficacy and glutaraldehyde shows the least effectiveness in sterilizing endodontic instruments. Endodontic files, as supplied by the manufacturers to the endodontists are sterile.The study concluded that N class autoclave could be considered as a best method of sterilization in clinical practice while comparing with the rest of the study groups.
Keywords: Sterilization, autoclave, rotary files.
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