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Category: Volume 08 Issue 06 June 2020
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Title: Isolation and Chracterization of Mesenchymal Stem Cells from Dental Pulp at a Tertiary Care Centre

Authors: Mathur Kusum, Yadav Deepti, Yawer Hussain Syed, Agrawal Swati, Meena Shivani

 DOI: https://dx.doi.org/10.18535/jmscr/v8i6.02

Abstract

 

Introduction: Stem cells have always been a fascinating area in research as they are the building blocks of human body. They have properties like self renewal, proliferation and multi- lineage differentiation. These indispensable properties have always brought a keen interest of researchers all around the world to use them for many diseases. There has always been search for newer and more reliable source to harvest stem cells from various parts of human body.

Aim: To study the growth pattern, visual analysis and quantification of mesenchymal stem cells isolated from dental pulp.

Materials and Methods: A descriptive type of observational study was done at SMS Medical College, Jaipur. The healthy tooth dental pulp extracted under all aseptic precautions and immediate culture of the pulp tissue done in stem cell laboratory. The culture cells were then evaluated to establish their mesenchymal nature by using immunocytochemistry technique.

Results:  Total of 10 healthy teeth were extracted and cultured in laboratory and yielded a mean cell count as 1.33X103 cells per D60 culture dish. The mean and median cell viability of the culture dishes came out to be 95%. The mean expression of CD73 in the study subjects was calculated as 94.50% , mean of expression of CD105 was calculated as 94.30%. CD271 was expressed in 42.20% of cells on an average. 

Conclusion: From our study we concluded that dental pulp is a significant source of stem cells with a mean viability 95%.These cells expressed a high percentage of mesenchymal markers like CD73 and CD105 establishing their mesenchymal nature.

Keywords: Mesenchymal stem cells, Dental pulp stem cells , Tissue culture.

References

  1. Ramalho SM, Willenbring H. On the origin of the term “stem cell”. Cell stem cell,june 7th 2007;1(1) :35-8.
  2. Polak JM, Bishop AE. Stem cells and tissue engineering: past, present, and future. Ann N Y Acad Sci. 2006;1068:352–66.
  3. Wada N, Gronthos S, Bartold PM. Immunomodulatory effects of stem cells. Peridontology2000. 2013; 63: 198-216.
  4. Bosio A, Bissels U, Miltenyi S. Regenerative medicine: From protocol to patient. 1st Netherlands: Springer, Dordrecht; 2011. Characterization and classification of stem cells. p. 149–67.
  5. Bongso A, Lee EH. Stem cells: From bench to bedside. 2nd Singapore: World Scientific; 2005.
  6. D’Aquino R, De Rosa A, Laino G, Caruso F, Guida L, Rullo R, et al. Human dental pulp stem cells: From biology to clinical applications. Journal of Experimental Zoology Part B Molecular and Developmental Evolution. July 2009; 312B: 408–15.
  7. Goldberg M, Smith AJ. Cells and extracellular matrices of dentin and pulp: A biological basis for repair and tissue engineering. Crit Rev Oral Biol Med. February 2004;15(1):13–27.
  8. Tirino V, Paino F, De Rosa A, Papaccio G. Somatic Stem Cells: Methods and Protocols. 1st New Jersey. Humana Press. 2012. Identification, isolation, characterization and banking of human dental pulp stem cells. Methods Mol Biol;879: 443–63.
  9. Kerkis I, Kerkis A, Dozortsev D, Stukart-Parsons GC, Gomes Massironi SM, Pereira LV, et al. Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers. Cells Tissues Organs. 2006;184 (3-4):105–16.
  10. Narang S, Sehgal N. Stem cells: A potential regenerative future in dentistry. Indian J Hum Genet. May 2012; 18(2):150-4.
  11. Malhotra N, Mala K. Regenerative endodontics as a tissue engineering approach: past, current and future. Aust Endod J. 2012; 38(3): 137-48.
  12. Sancherz- Lara P A, Zhao H, Bajpai R, Abdelhamid A, Warburton D. Impact of Stem Cells in Craniofacial Regenerative Medicine. Front Physiol. 2012; 3: (188): 1-9.
  13. Gronthos S, Mankani MH, Brahim JB, Robey PG, Shi S. Postnatal human dental pulp stem cells in vitro and in vivo. Proc Natl Acad Sci USA. 2000;97(25): 13625-30.
  14. Tsai AI, Hong HH, Lin WR, Fu JF, Chang CC, Wang IK, et al. Isolation of mesenchymal stem cells from human deciduous teeth pulp. Biomed Res Int.  March 2017; Article ID-2851906, 9 pages.
  15. Mohamadreza BE, Nazarian H, Shariati M, Vahabi S, Falahi F. Isolation and in vitro characterization of mesenchymal stem cells derived from the pulp tissue of human third molar tooth. Iran J Med Sci. September 2010; 35(3): 216-25.
  16. Samiei M, Aghazadeh M, Movassaghpour AA, Fallah A, Aminabadi NA, Mahdi S et al. Isolation and characterization of dental pulp stem cells from primary and permanent teeth . J Am Sci. 2013;9(12): 153-7.
  17. Luisi SB, Sant M, Filho A, Pranke P. Isolation, immunophenotypic characterization and pluripotency of dental pulp stem cells. Dent Oral Craniofac Res. 2017;3(5): 1-3.
  18. Naz S, Khan FR, Zohra RR, Lakhindi S, Khan MS, Mohammed N. isolation and culture of dental pulp stem cells from permanent and deciduous teeth. Pak J Med Sci. 2019 August;35(4):997-1002.

Corresponding Author

Yadav Deepti

Resident, Department of Pathology, SMS Medical College, Jaipur, Rajasthan, India