Details
Category: Volume 08 Issue 02 February 2020
Hits: 1075

Title: A Serological study on Leptospirosis during post flood epidemic in Kerala

Authors: Dr Neethu John, Dr Tanya Tonny Mampilly, Dr Chris Mary Antony, Dr Keerthy Bose, Dr Kiran.T Jose, Dr Ria Catherine Vincent, Dr J. Lancy

 DOI: https://dx.doi.org/10.18535/jmscr/v8i2.124

Abstract

   

Leptospirosis is an emerging and reemerging bacterial zoonosis caused by Spirochaetes belonging to the genus Leptospira. Various serological tests are available for the confirmatory diagnosis of Leptospirosis in the laboratory. IgM ELISA is commonly done in the first week of fever in most of the laboratories.PCR has been a major advance but it is available only in a few centres in Kerala. We had an epidemic in the first week of September 2018 following the floods. A total number of 373 blood samples collected from suspected cases of Leptospirosis were subjected to serological tests in the 24 hour Serology laboratory at Government Medical college Hospital, Ernakulam. IgM ELISA for Leptospira was found to be positive in 27 cases (7.2%).Blood samples and urine samples were sent to NIV Alappuzha for PCR testing. Among the 111 serum and urine samples tested, 9 serum samples and  urine sample were positive for Leptospira by PCR. Mortality rate was less than 1 % in suspected cases and 11.11% in PCR positive cases of Leptospirosis.

Keywords: Leptospirosis, ELISA, PCR

References

  1. S Mullan et al ,Polymerase Chain Reaction,An important tool for early diagnosis of Leptospirosis cases. Journal of Clinical and Diagnostic Research 2016 Dec,Vol10(12): DC 08-DC11
  2. R Kanimozhi et al, A Serological study of Leptospirosis in Chennai, International journal of Research in Medical Sciences.
  3. France G Terpan, Outbreak of Leptospirosis during a can yoning weekend, t et al,2017,vol:66 supplement 5,July 2018 Pages S361
  4. G Vimala, Mary Josephine Rani, Raja Gopal, Leptospirosis in Vellore :A clinical and Serological study. International Journal of Microbiology Vol 2014 10.1155/2014/643940
  5. V Chauhan, Mahesh, Panda et al, Profile of patients of Leptospirosis in Sub Himalayan region of North India. JAPI, June 2010 Vol :58:354-356
  6. Chaudry, Das, Premlatha et al, Serological and molecular approaches for diagnosis of Leptospirosis in a tertiary care hospital in North India: A 10 year study, Indian Journal of Medical Research,2013 April 137(4):785-790
  7. Al-Share, T Amilasan, Mugenvijile et al, Outbreak of Leptospirosis after flood, the Philippines,2009,Emerging Infectious diseases Vol:18,No.1, January 2012 pages 91-93
  8. Karande S, Kulkarni H, Kulkarni M et al, Leptospirosis in children in Mumbai slums, Indian Journal of Paediatrics, 2002,69:855-858
  9. M Nizamuddin et al, Early diagnosis of human Leptospirosis by Antigen detection in blood.Indian Journal of Medical Microbiology,Oct 2006,Vol 24:No.4
  10. Smita B Shekatkar, Belgode N et al ,Clinical and Serological evaluation of Leptospirosis in Puducherry, India.. Journal of Infection in Developing countries, 2010:4(3):139-143
  11. Harrisons Principles of medicine 2008,Vol 1,17th Edition Pages 1048-51.

Corresponding Author

Dr J.Lancy

Professor, GMC, Ernakulam, India