Title: Anti-Oxidant and Anti-Cancer Potential of Siddha Medicine Thayiriya Vendhan Chendhuram (TVC) using MTT Assay against MCF- 7 (Human Breast Adenocarcinoma)
Authors: Vaniswari D.S, Arunachalam K
DOI: https://dx.doi.org/10.18535/jmscr/v7i5.111
Abstract
Objective: The objective of this study was to evaluate the invitro anti oxidant potential and anticancer efficacy of Thayiriya vendhan chendhuram -TVC.
Methods: We have evaluated scientifically TVC for its anti cancer efficacy in human breast adenocarcinoma through in vitro cell lines studies. The viability of cells was evaluated by direct observation of cells by Inverted phase contrast microscope and followed by MTT assay method .In this study, anti oxidant potential of TVC was also evaluated with gallic acid as comparison.
Results and Conclusion: From the result of the present in-vitro study it was concluded that the test drug TVC possess considerable anti-oxidant and anti-cancer activity. The study is the first step in the scientific validation of TVC for anti oxidant and anticancer potential and in use against breast adenocarcinoma.
Keywords: Breast cancer, Siddha oncology, Anti oxidant drug, Thayiriya vendhan chendhuram, herbo metallic drug.
References
- Gupta AK and Tandon N. Reviews on Indian Medicinal Plants. New Delhi: Indian Council of Medical Research, 2004.
- Izevbigie EB Discovery of water-soluble anticancer agents (edotides) from a vegetable found in Benin City, Nigeria. Exp Biol Med (Maywood), 2003; 228:293-298.
- Om Prakash, Amit Kumar, Pawan Kumar, and Ajeet, “Anticancer Potential of Plants and Natural Products: A Review.” American Journal of Pharmacological Sciences 1, no. 6 2013; 104-115. doi: 10.12691/ajps-1-6-1.
- Krishnamurthi K. Screening of natural products for anticancer and antidiabetic properties. Health Administrator. XX ,2000 ;(1&2): 69.
- Srinivas; A.J Afolayan. Current Science, 2007; 92, 906-8.
- Kathiresan; N.S Boopathy; S. Kavitha. Natural Product Radience, 2006; 5, 115-19.
- Norn S, Permin H, Kruse E, Kruse PR. Mercury a major agent in the history of medicine and alchemy. Dan Medicinhist Årboq. 2008;36:21–40. [PubMed] [Google Scholar]
- Frezza M, Hindo S, Chen D, et al. Novel metals and metal complexes as platforms for cancer therapy. Curr Pharm Des. 2010;16(16):1813–1825. [PMC free article] [PubMed] [Google Scholar]
- Halliwell B. Biochemistry of oxidative stress. Biochem Soc Trans. 2007; 35:1147–
- Sies H. Oxidative stress: oxidants, antioxidants. Exp Physiol. 1997; 82:291–
- Halliwell B, Gutteridge JMC: Formation of thiobarbituric acid reactive substances from deoxyribose in the presence of iron salts: the role of superoxide and hydroxyl radicals. FEBS Lett. 1981, 128: 347-352.
- Gulcin I: Antioxidant activity of food constituents:an overview. Arch Toxicol. 2012, 86: 345-391.
- Gocer H, Gulcin I: Caffeic acid phenethyl ester (CAPE): correlation of structure and antioxidant properties. Int J Food Sci Nutr. 2011, 62: 821-825.
- WHO PEN Protocol 4.1 Assessment and referral of women with suspected breast cancer at primary health care pdf,59kb
- Siddha Maruthuva Chinthamani Palm leaf manuscript of Tamil Medicine, published by Department of Indian Medicine and Homeopathy, Chennai, First edition 2008, Page.no:17
- Alugoju Phaniendra. Free Radicals: Properties, Sources, Targets, and Their Implication in Various Diseases. Indian J Clin Biochem. 2015 Jan; 30: 11– 26