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Category: Volume 06 Issue 08 August 2018
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Title: To study association of trp 53 arg 72 pro polymorphic variant with breast cancer-a case control study in north Indian population

Authors: Pankaj Kumar Kannaujiya*, Samir Misra, Gitika Nanda Singh, Vinod Jain

 DOI:  https://dx.doi.org/10.18535/jmscr/v6i8.168

Abstract

Objectives: The single nucleotide polymorphism (SNP) at Trp53arg72 pro genotypes is found to be associated with breast cancer, with variable results, the aim of this study is to evaluate the frequency of   SNP at Trp 53 codon 72 and its association with breast cancer in north Indian population.

Methods: 30 patients with breast cancer were compared with 30 controls. Genotype and allele distribution of Trp53 codon 72 where determined using polymerase chain reaction- restriction fragment length polymorphism method.

Results: The genotypic distribution of arg/arg, arg/pro, pro/pro among cancer patients vs  controls were 53.33%, 33.33%, 13.33% and 56.7%, 26.7%, 16.7% respectively (p=0.83).

Conclusion: though the difference in genotypic distribution was not significant, we found in the higher number of heterozygous genotypes (arg/pro) in cancer tissue suggesting, heterozygous (arg/pro) genotype may predispose women to breast cancer. This necessitates conducting the study including more patients.

References

  1. Vogelstein B, Lane D, Levine AJ: Surfing the p53 network. Nature. 2001, 408: 307-310. 10.1038/35042675.View Article Google Scholar
  2. Zhang H, Somasundaram K, Peng Y, Tian H, Zhang H, Bi D, Weber BL, El-Deiry WS: BRCA1 physically associates with p53 and stimulates its transcriptional activity. Oncogene. 1998, 16: 1713-1721. 10.1038/sj/onc/1201932. PubMed View Article Google Scholar
  3. Catteau A, Harris WH, Xu CF, Solomon E: Methylation of the BRCA1 promoter region in sporadic breast and ovarian cancer: correlation with disease charact-eristics. Oncogene. 1999, 18: 1957-1965. 10.1038/sj/onc/1202509.PubMed View Article Google Scholar
  4. Dumont P, Leu JI, Della Pietra AC et al. The codon 72 polymorphic variants of p53 have markedly different apoptotic potential. Nat Genetics. 2003; 33(3): 357- 65.
  5. Sinilnikova OM, Antoniou AC, Simard J, Healey S, Léoné M, Sinnett D, Spurdle AB, Beesley J, Chen X, KConFab, Greene MH, Loud JT, Lejbkowicz F, Rennert G, Dishon S, Andrulis IL OCGN, Domchek SM, Nathanson KL, Manoukian S, Radice P, Konstantopoulou I, Blanco I, Laborde AL, Durán M, Osorio A, Benitez J, Hamann U, Hogervorst FB, van Os TA, Gille HJ HEBON, et al. The TP53 Arg72Pro and MDM2 309G4T polymorphisms are not associated with breast cancer risk in BRCA1 and BRCA2 mutation carriers. Br J Cancer. 2009;101:1456–1460. [PMC free article]  [PubMed]
  6. Lum SS, Chua HW, Li H, Li WF, Rao N, Wei J, Shao Z, Sabapathy K. MDM2 SNP309 G allele increases risk but the T allele is associated with earlier onset age of sporadic breast cancers in the Chinese population. Carcinogenesis. 2008;29:754–761. doi: 10.1093/carcin/bgn024.  [PubMed] [Cross Ref]
  7. Zhou Y, Li N, Zhuang W, et al. P53 codon 72 polymorphism and gastric cancer: a meta-analysis of the literature. Int J Cancer. 2007;121:1481–1486.  [PubMed]
  8. Suspitsin EN, Buslov KG, Grigoriev MY, et al. Evidence against involvement of p53 polymorphism in breast cancer predispose-tion. Int J Cancer. 2003;103:431–433.  [PubMed]
  9. Noma C, Miyoshi Y, Taguchi T, Tamaki Y, Noguchi S. Association of p53 genetic polymorphism (Arg72Pro) with estrogen receptor positive breast cancer risk in Japanese women. Cancer Lett. 2004;210: 197–203.  [PubMed]
  10. Franeková M, Zúbor P, Stanclová A, Dussan CA, Bohusová T, Galo S, Dobrota D, Kajo K, Péc M, Racay P. Association of p53 polymorphisms with breast cancer: a case-control study in Slovak population. Neoplasma. 2007;54:155–161.  [PubMed]
  11. Ohayon T, Gershoni-Baruch R, Papa MZ, Distelman Menachem T, Eisenberg Barzilai S, Friedman E. The R72P P53 mutation is associated with familial breast cancer in Jewish women. Br J Cancer. 2005;92:1144–1148. [PMC free article]  [PubMed]
  12. Ma H, Hu Z, Zhai X, Wang S, Wang X, Qin J, Chen W, Jin G, Liu J, Gao J, Wang X, Wei Q, Shen H. Joint effects of single nucleotide polymorphisms in P53BP1 and p53 on breast cancer risk in a Chinese population. Carcinogenesis. 2006;27:766–771.  [PubMed]
  13. Damin AP, Frazzon AP, Damin DC, et al. Evidence for an association of TP53 codon 72 polymorphism with breast cancer risk. Cancer Detect Prev. 2006;30:523–529.  [PubMed]
  14. Mabrouk I, Baccouche S, El-Abed R, Mokdad-Gargouri R, Mosbah A, Saïd S, Daoud J, Frikha M, Jlidi R, Gargouri A. No evidence of correlation between p53 codon 72 polymorphism and risk of bladder or breast carcinoma in Tunisian patients. Ann NY Acad Sci. 2003;1010:764–770.  [PubMed]
  15. Tommiska J, Eerola H, Heinonen M, Salonen L, Kaare M, Tallila J, Ristimäki A, von Smitten K, Aittomäki K, Heikkilä P, Blomqvist C, Nevanlinna H. Breast cancer patients with p53 Pro72 homozygous genotype have a poorer survival. Clin Cancer Res. 2005;11:5098–5103.  [PubMed]
  16. Khadang B, Fattahi MJ, Talei A, Dehaghani AS, Ghaderi A. Polymorphism of TP53 codon 72 showed no association with breast cancer in Iranian women. Cancer Genet Cytogenet. 2007;173:38–42.  [PubMed]
  17. Beckman G, Birgander R, Sjalander A, Saha N, Holmberg PA, Kivela A, Beckman L, 1994. Is p53 polymorphism maintained by nature selection? Human Heredity, 44:266-270.
  18. IARC TP53 Database, 2010. http://wwwp53.iarc.fr/Polymorphism.html
  19. Suresh K, Venkatesan R, Chandirasekar R, Kumar BL, Sasikala K. Association of Trp53 arg72pro polymorphic variants with breast cancer – a case control study in south Indian population. Biol Med. 2011;3(1):15–22.
  20. jing hou, yuan jiang,1 wenru tang,2 and shuting jia2 .p53 codon 72 polymorphism and breast cancer risk: A meta-analysis; Exp Ther Med. 2013 May; 5(5): 1397–1402.
  21. Meire Luzia Gonçalves, Sarah Moreira Borja, Jacqueline Andréia Bernardes Leão Cordeiro, Vera Aparecida Saddi, Flávio Monteiro Ayres, Cesar Augusto Sam Tiago Vilanova-Costa, and Antonio Márcio Teodoro Cordeiro Silva, Association of the TP53 codon 72 polymorphism and breast cancer risk: a meta-analysis,;Springerplus. 2014; 3: 749.

Corresponding Author

Pankaj Kumar Kannaujiya

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