Title: A Clinico-Mycological Study of Human Dermatophytosis in Chitradurga, Krnataka, India

Authors: G.L. Aruna, Ramalingappa B.

 DOI:  https://dx.doi.org/10.18535/jmscr/v5i7.236

Abstract

Dermatophytosis is a superficial fungal infection. It is caused by keratinophilic fungi called dermatophytes. The aim of this work is to detect the most prevalent form of dermatphytosis and the most prevalent dermatophytic fungi from clinical samples of human dermatophytosis. A total of 135 clinical samples include skin, hair and nail samples collected from outpatient Depatment of Dermatology, Govt. hospital, Chitradurga. They were processed by direct microscopy of KOH mount and culture method using Sabouraud dextrose agar medium (SDA) with chloramphenicol and Dermatophyte Test Medium (DTM). Among different dermatophytic infections studied, the tinea corporis was most prevalent; it was of 52.68%, followed by tinea cruris 34.40%. The infection was more in men (74.%) than women (26%). Among four age groups studied, it was more prevalent in 21-40 age group. Culturing and identification of positive clinical samples revealed the presence of dermatophytes like Trichophyton rubrum, Trichophyton mentagrophyte, Epidermophyton floccosum, Microsporum canis and Microsporum gypseum, non dermatophytes such as yeasts like Candida albicans and molds like A. niger. Among the dermatophytes isolated the most prevalent species found was Trichophyton rubrum. It constituted 67.74% of total dermatophyte isolates.

Keywords: Dermatophytosis, DTM, KOH mount and Keratinophilic fungi.

References

  1. Grapple FS, Bishop TC and Blank F. Immunology of Dermatophytes and Dermatophytosis. Bacteriological Reviews. 1974, 38:222-250.
  2. Weitzman I and Summerbell RC. The Dermatophytes. Clinical Microbiological Reviews. 1995, 8: 240-259.
  3. Ndako JA, Osemwegie OO, Spencer THI, Olopade BK, Yunusa GA and Banda J. Prevalence of Dermatophytes and other associated Fungi among school children. Global Advanced Research Journal of Medicine and Medical Sciences. 2012, 1:49-56.
  4. Kaufman G, Berdicevsky I,  Woodfolk JA  and Horwitz BA. Markers for Host-Induced Gene Expression in Trichophyton Infection and Immunity, 2005, 73:6584–6590.
  5. Koga T, Duan H, Urabe K, Furue M, Maidashi and Higashi-Ku. Immunohisto-chemical detection of interferon-gamma-producing cells in dermatophytosis. Europian Journal of Dermatology. 2010, 11:105-107.
  6. Woodfolk JA. Allergy and Dermatophytes. A Clinical Microbiology Reviews. 2005, 18:30-43.
  7. Madhavi S, Rama Rao MV and Jyothsna K. Mycological study of Dermatophytosis in rural population. Annals of Biological Research. 2011,2:88-93.
  8. Gharachorlou A, Hashemi SJ, Gharachorlou S and Ghalekandi JG. Study of relationship among Testosterone and Dermatophytosis due to Microsporium gypseum. Advances in Environmental Biology. 2011, 5:2166-2168.
  9. Balakumar S, Rajan S, Thirunalasundari T and Jeeva S. Epidemiology of dermato-phytosis in and around Tiruchirapalli, Tamilnadu, India. Asian Pacific Journal of Tropical Disease. 2012, 2:286-289.
  10. Cortez CAA, de Souza JVB, Sadahiro A and de Oliveira JAA. Frequency and aetiology of dermatophytosis in children age 12 and under in the state of Amazonas, Brazil. Revista Iberoamericana de Micologia. 2012, 29:223-226.
  11. Zuzarte M, Gonçalves MJ, Canhoto J and Salgueiro, L. Antidermatophytic activity of essential oils. In: Mendez-Vilas, edit-ors. Science against microbial pathogens: communicating current research and technological advances. Formatex Spain, 2011. pp 1167-1178.
  12. Sharma M and Sharma R. Profile of dermatophytic and other fungal infections in Jaipur. Indian Journal of Microbiology. 2012, 52: 720-740.
  13. Doddamani PV, Harshan KH, Kanta RC, Gangane R and Sunil KB. Isolation, Identification and Prevelance of Dermatophytes in Tertairy Care Hospital in Gulbarga District. People’s Journal of Scientific Research. 2013, 6:10-13.
  14. Havlickova B, Czaika VA and Friedrich M. Epidemiological trends in skin myc-oses worldwide. Mycoses. 2008, 51:2-15.
  15. Cortez CAA, de Souza JVB, Sadahiro A and de Oliveira JAA. Frequency and aetiology of dermatophytosis in children age 12 and under in the state of Amazonas, Brazil. Revista Iberoamericana de Micologia. 2012, 29:223-226.
  16. Seebacher C, Bouchara JP and Mignon B. Updates on the Epidemiology of Dermatophyte Infections. Mycopathologia. 2008, 166:335–352.
  17. Dehghan M, Hajian S, Alborzi N, Borgheyee A and Noohi AH. Clinico-mycological Profiles of Dermatophytosis in Gorgan, North of Iran. Iranian Journal of Dermatology. 2009,12:13-15.
  18. Mathur M, Kedia SK and Ghimire RBK. “Epizoonosis of Dermatophytosis”: A Clinico - Mycological Study of Dermato-phytic Infections in Central Nepal. Kath-mandu University Medical Journal. 2012, 37:30-33.
  19. Hayette MP and Sacheli R. Dermatophytosis, Trends in Epidemiology and Diagnostic Approach. Current Fungal Infection Reports. 2015, 9:164-179.
  20. Hanumanthappa SB, Sarojini SB and Shilpashree SB, Muddapur SB. Clinicomycological study of 150 cases of dermatophytosis in a tertiary care hospital in South India. Indian Journal of Dermatology. 2012, 57: 322-3.
  21. Venkatesh VN and Kotian N. Dermatophytosis: A Clinico-mycological profile from a tertiary care hospital. Journal of International Medicine and Dentistry. 2016, 3:96-102.
  22. Kaur R, Kashyap B and Bhalla P. Onychomycosis-epidemiology, diagnosis and management. Indian Journal of Medical Microbiology. 2012, 26:108-16.
  23. Golia S, Hittinahalli V, Vasudha CL, Sangeetha L, Mohan KTM and Syrti C. A study on the mycological profile of onychomycosis. Journal of Evolution of Medical and Dental Sciences. 2012, 1:1247-1255.
  24. Ramaraj V, Vijayaraman RS, Rangarajan S and Kindo AJ. Incidence and prevalence of dermatophytosis in and around Chennai, Tamilnadu, India. International Journal of Research in Medical Sciences.2016, 4:695-700.

Corresponding Author

Dr Ramalingappa B.

Associate Professor, Department of Studies in Microbiology, Davangere University

Email: This email address is being protected from spambots. You need JavaScript enabled to view it., Mob: 9901120803