Production of Levansucrase from a Soil Isolate Bacillus Subtilis (DQ922949)

Levansucrase is a fructosyl transeferase, which brings hydrolytic breakdown of sucrose to glucose
and fructose. The fructose was polymerized to form levan. Levan a biologically active polymer has
potential application in the field of food as a low calorie sweetener, cosmetics and in
pharmaceuticals as antiviral, anti- diabetic and antitumor agent. In this context the present work
brought screening of 11 soil isolates for levan production. Maximum levan producing isolate
(25.8g/L) was identified by 16S rRNA as Bacillus subtilis (DQ922949). Maximum enzyme activity
was found to be 1250 U/ml. The optimum temperature and pH for levansucrase activity were 600C
and 7.0 respectively. Enzyme showed maximum activity at 0.8M sucrose concentration. Different
metal ions K+
, Mg+2, Fe+3, Mn+
, Hg+2and Ca+2 showed inhibitory effect on enzyme activity. The
enzyme was partially purified by ammonium sulfate precipitation